This repository contains the code and analysis instructions for the B7-H3 project.
Dual Functions of B7-H3–MAPK Signaling Orchestrate Tumor Immunosuppression by Regulating the Plasticity of MDSCs and Fibroblasts
| Analysis Part |
Purpose |
Link |
| PbPPCd276 analysis |
Single-cell RNA sequencing (scRNA-seq) analysis of prostate tumors from PB-Cre;PtenL/L;Trp53L/L (PbPP) and PB-Cre;PtenL/L;Trp53L/L;Cd276L/L (PbPPCd276) genetically engineered mouse models (GEMMs). |
Code |
| Treatment analysis |
Evaluation of therapeutic efficacy by combining B7-H3 monoclonal antibody (mAb) treatment with MEK inhibitor therapy. |
Code |
| Human data analysis |
Analysis of publicly available human single-cell RNA-seq datasets from the Gene Expression Omnibus (GEO), accession GSE274229. |
Code |
| Other functions |
Supporting scripts for analyses, including cell type composition comparison and marker gene heatmap visualization. |
Cell Type Composition Comparison, Marker Gene Heatmap |
- Each analysis step is documented in the corresponding code link.
